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. 2015 Sep 15;12:141. doi: 10.1186/s12985-015-0369-2

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© Deng et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Analysis of viral cell-to-cell movement by confocal microscopy. Leaves of Nicotiana benthamina agro-infiltrated with the wild type parental virus TuMV::6 K2-GFP (positive control), the replication-defective mutant NIbΔGDD (negative control) and CI mutants were observed under a confocal microscope 4 days post infiltration. a Multicellular infection of TuMV::6 K2-GFP in the inoculated leaf. b Single cell expression of the replication-defective mutant NIbΔGDD (resulting from the 35S promoter). c Single cell infection of the TuMV::6 K2-GFP CIm1 mutant. Scale bar, 100 μm