Fig. 6.

Cyp7a1 and Cy27a1 mRNA expression in the liver samples of trained and sedentary C57BL/6N wild type mice by quantitative real-time PCR. Using reverse transcriptase, cDNA was synthetized from 2 μg from total RNA isolated from the liver samples of trained (n = 6 - black bars) and sedentary (n = 6 - white bars). The TaqMan gene expression assays used were Mm00484150_m1 (Cyp7a1) and Mm00470430_m1 (Cyp27a1) and quantification was normalized to the endogenous Actb (Mm00607939_s1). Real-time PCR was performed using Gene Expression Master Mix (Applied Biosystems). Data analysis was performed using 2^-ΔΔCt method. Data are expressed as mean values ± standard error