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. 2015 Sep 15;8:455. doi: 10.1186/s13071-015-1087-8

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© Habtewold et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Differential staining of live and dead ookinetes and bacteria in the mosquito midgut. a Heat killed ookinetes an overnight ookinete culture stained with a mixture of PMA and SYBR solution. b Live and dead parasites from an overnight ookinete culture stained with a mixture of PMA and SYBR solution. c Live and dead P. berghei ookinetes in the mosquito midgut epithelium at 48 hpi. d Live and dead bacteria present in midgut contents. Live cells are stained with SYBR green (white arrowheads), while dead cells are stained with PMA (red arrowheads)