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N279K tau mutant alters intracellular vesicle markers and their distributions in NSCs. a Immunostaining of Flotillin-1, EEA1 and LAMP-1 in control and N279K NSCs. b-f Western blot analysis of Flotillin-1, CHMP2B, EEA1 and LAMP-1 in control and N279K NSCs. Quantification of Western blot densitometry of Flotillin-1 c, EEA1 d, CHMP2B e and LAMP-1 levels f. Data are mean ± SEM from three independent experiments. *p < 0.05; **p < 0.01
