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. Author manuscript; available in PMC: 2015 Sep 17.
Published in final edited form as: J Surg Res. 2009 May 3;156(1):26–31. doi: 10.1016/j.jss.2009.03.072

Figure 3.

Figure 3

Time course of IL-8 mRNA induction by EGF and /or BBS. A) Autoradiography of Northern blots demonstrates steady state levels of IL-8 mRNA after treatment with EGF (1 ng/ml), BBS (100nM), or both EGF and BBS. Total RNA (15 μg/lane) was isolated from MDA-MB-231 cells at the indicated time points after treatment. RNA was resolved on a 1% agarose-formaldehyde gel, blotted onto a Hybond-N+ membrane, and hybridized with a [α32P]dATP-labeled cDNA probe. Equal loading and transfer was verified by rehybridizing the blot with a radiolabeled probe for 18S RNAse. B) Quantitative real-time PCR analysis of IL-8 mRNA levels showing synergistic increase at 4 h. As a negative control, mRNA from SK-BR-3 cells was used.