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. Author manuscript; available in PMC: 2015 Sep 17.
Published in final edited form as: J Surg Res. 2009 May 3;156(1):26–31. doi: 10.1016/j.jss.2009.03.072

Figure 4.

Figure 4

Effects of BBS on SK-BR-3 cells transfected with GRP-R. A, C) Time-course of the BBS-induced change in [Ca2+]i in SK-BR-3 cells transfected with empty vector. Psuedo-color image of cells loaded with Fura-2, a Ca2+sensitive dye, before and after treatment with BBS (100 nM) shown below. B, D) Time-course of the BBS-induced change in [Ca2+]i in SK-BR-3 cells stably transfected recombinant GRP-R. Psuedo-color image of cells loaded with Fura-2, demonstrating a BBS-stimulated response shown below. E) Cells were allowed to migrate in chemotaxis chamber assays as described in “Materials and Methods”. The experiment was performed in triplicate and repeated two times. A one-way ANOVA test demonstrated significant differences between the groups and Dunn's Multiple Comparison test was used to assess significance between paired groups. Cells expressing GRP-R demonstrated significantly more migration toward BBS in the lower chamber compared with either vector transfected cells or GRP-R transfected cells in the absence of chemoattractant. F) Time course of human IL-8 mRNA expression by SK-BR-3-GRPR cells stimulated with BBS (100 nM). IL-8 mRNA expression was induced after 2 h of treatment with BBS, and decreased by 4 h.