Table 2. Primers used for qPCR.
Gene | Sequence (5’– 3’) | |
---|---|---|
18S rRNA | Fw | GTGGAGCGATTTGTCTGGTT |
Rv | ACGCTGAGCCAGTCAGTGTA | |
β-ACTIN | Fw | ACTTCGAGCAAGAGATGGCC |
Rv | CACATCTGCTGGAAGGTGG | |
β-GLOBIN | Fw | CGGCGGCGGGCGGCGCGGGCTGGGCGGCTTCATCCACGTTCACCTTG |
Rv | GCCCGGCCCGCCGCGCCCGTCCCGCCGGAGGAGAAGTCTGCCGTT | |
c-MET | Fw | GGGTCGCTTCATGCAGGTTGTGGT |
Rv | ATGGTCAGCCTTGTCCCTCCTTCA | |
COLLAGEN I | Fw | AAGGTCATGCTGGTCTTGCT |
Rv | GACCCTGTTCACCTTTTCCA | |
COLLAGEN IV | Fw | ACTCTTTTGTGATGCACACCA |
Rv | AAGCTGTAAGCGTTTGCGTA | |
FN1 | Fw | AGACCCCAGGCACCTATCAC |
Rv | TCGGTCACTTCCACAAACTG | |
GATA-4 | Fw | ACCTGGGACTTGGAGGATAGCAAA |
Rv | TCCCATCAGCGTGTAAAGGCATCT | |
HGF | Fw | ACGCTACGAAGTCTGTGACA |
Rv | CAAGAGTATAGCACCATGGC | |
HMBS | Fw | GGCAATGCGGCTGCAA |
Rv | GGGTACCCACGCGAATCAC | |
hTERT | Fw | GACGTGGAAGATGAGCGTG |
Rv | GACGACGTACACACTCATC | |
IGF-1 | Fw | CTTCAGTTCGTGTGTGGAGACAG |
Rv | CGCCCTCCGACTGCTG | |
IGF-1R | Fw | CTCCTGTTTCTCTCCGCCG |
Rv | ATAGTCGTTGCGGATGTCGAT | |
IGF-2R | Fw | GAGGGAAGAGGCAGGAAAG |
Rv | TGTGGCAGGCATACTCAG | |
ITGA1 | Fw | GGTTCCTACTTTGGCAGTATT |
Rv | AACCTTGTCTGATTGAGAGCA | |
ITGB4 | Fw | AGACGAGATGTTCAGGGACC |
Rv | GGTCTCCTCTGTGATTTGGAA | |
MEF-2C | Fw | GACTGTGAGATTGCGCTGAT |
Rv | CGTCTCCACGATGTCTGAGT | |
MEF-2D | Fw | CCCCTGCTGGAGGACAAGTA |
Rv | CCCCTGCTGGAGGACAAGTA | |
MMP-2 | Fw | GGCCCTGTCACTCCTGAGAT |
Rv | GGCATCCAGGTTATCGGGGA | |
MMP-14 | Fw | CCCTATGCCTACATCCGTGA |
Rv | TCCATCCATCACTTGGTTAT | |
NKX2.5 | Fw | CGCCGCTCCAGTTCATAG |
Rv | GGTGGAGCTGGAGAAGACAGA | |
SDF-1 | Fw | GGTGGAGCTGGAGAAGACAGA |
Rv | CAGCCGGGCTACAATCTGAA | |
CXCR4 | Fw | TGACGGACAAGTACAGGCTGC |
Rv | CCAGAAGGGAAGCGTGATGA | |
Telomere | Fw | ACACTAAGGTTTGGGTTTGGGTTTGGGTTTGGGTTAGTGT |
Rv | TGTTAGGTATCCCTATCCCTATCCCTATCCCTATCCCTAACA | |
VE-cad 2 | Fw | GGCATCTTCGGGTTGATCCT |
Rv | CCGACAGTTGTAGGCCCTGTT | |
VEGF | Fw | TGCAGATTATGCGGATCAAACC |
Rv | TGCATTCACATTTGTTGTGCTGTAG | |
VEGFR-1 | Fw | CAGGCCCAGTTTCTGCCATT |
Rv | TTCCAGCTCAGCGTGGTCGTA | |
VEGFR-2 | Fw | CCAGCAAAAGCAGGGAGTCTGT |
Rv | TGTCTGTGTCATCGGAGTGATATCC | |
VLA-4 | Fw | ATCTGACTCTGCCTTCAT |
Rv | CATTCCTCACCATCACTG |