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. 2015 Sep 16;10(9):e0138085. doi: 10.1371/journal.pone.0138085

Fig 4. KP1019 induces a pre-anaphase cell cycle delay.

Fig 4

A and B. Cells arrested in the G1 phase of the cell cycle by mating pheromone were released in the absence (0 mg/mL KP1019) and presence (80 mg/mL KP1019) of drug. Pds1-myc (A) and budding index (B) were measured every 20 minutes after release from G1. A. Pds1-myc epitope tagged protein expression levels assayed as cells progress through the cell division cycle. Antibody against the Nop1 protein is used as loading control. Normalized Pds1 band intensities are represented below each blot. B. Budding index (% of unbudded cells in the population) of cells used to measure Pds1 levels as cells progress through the cell division cycle. C. Distance between SPB in large budded cells that are untreated (blue bar) or treated with 80 mg/mL KP1019 for three hours (red bar) were determined as described in Materials and Methods. The average of three replicates is presented with 2XSE error bar.