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. Author manuscript; available in PMC: 2015 Sep 17.
Published in final edited form as: Urol Oncol. 2010 Oct 2;30(5):635–645. doi: 10.1016/j.urolonc.2010.06.011

FIGURE 2.

FIGURE 2

FIGURE 2

Binding specificity of PLZ4. (A) PLZ4 could bind to bladder tumor cells, but not to normal urothelial cells or other confounding cells. A whole cell binding assay was performed to determine cell binding of PLZ4. To detect any weak binding, cells and beads were incubated for at least 4 hours while rotating in culture. PLZ4 bound to 3 human bladder TCC cell lines: 5637 (a), T24 (b) and TCCSUP (c), but not to normal human urothelial cells (d). The heterogeneity of cell size of normal urothelial cells in Panel d suggests the presence of cells from basal (small cells) to suprabasal layers of urothelium. No binding of PLZ4 was observed with whole blood cells (e), PBMC (f), normal fibroblasts (g). PLZ4-coated beads could bind to 5637 bladder cancer cells after 4 hours of incubation in urine with pH = 6.0 (h). The bead diameter is approximately 90μm. (B) PLZ4 specifically binds to 5637 bladder TCC cell lines. ELASA was performed to determine the binding specificity. The lower panel table showed the statistical analysis (P value) of ELASA results between 5637 and other cell lines (P <0.05).