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. 2015 Sep 8;10:5687–5700. doi: 10.2147/IJN.S87174

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© 2015 Gehrmann et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Target specificity of antibody-coated gold nanoparticle (Au-Hsp70) uptake.

Notes: 4T1 mouse mammary carcinoma cells were incubated with antibody-coated nanoparticles (1 µg/mL) for 24 hours at 37°C. (A) In 100% of the 4T1 cells with a wild-type expression of Hsp70, cmHsp70.1-coated nanoparticles could be detected as black dots (arrows). (B) In transfected cells, 4T1 Hsp70−/− cells with knocked down Hsp70, no uptake of nanoparticles could be detected. (C) The successful knock down of Hsp70 in 4T1 Hsp70−/− cells was verified by Western blotting compared with the expression of Hsp70 in the cytosol of 4T1 wt cells. Images are representative for at least three independent experiments. (A and B bright field, objective 40×, scale bar 20 µm.)

Abbreviation: wt, wild-type.