Figure 6.

Histochemistry for ALP, immunohistochemistry for RUNX2, and immunofluorescence for OCN in paraffin sections from femur.

Notes: (A) ALP expression was significantly increased in OVX + BN mice (D) that received BN (10 mg/kg per day) for 3 months when compared with the OVX group (D). Histological sections from the sham, OVX, and OVX + BN groups were incubated with RUNX2 (B) and OCN (C) antibody. Expression of RUNX2 (E) and OCN (F) around trabecular bone decreased after ovariectomy but was rescued with BN. Each group contained ten mice. The black arrows indicate ALP positive cells (A). *P<0.05 versus OVX group. The values shown are the mean ± standard error of the mean of data from independent experiments (D–F).

Abbreviations: BN, baicalein; OCN, osteocalcin; RUNX2, runt-related transcription factor 2.