Table 1.
Primer sequences used in RT-PCR and quantitative PCR
| Target gene | GenBank accession no | Sequences (5′–3′) | Product size |
|---|---|---|---|
| GAPDH | NM_017008.4 | Forward, CAGGGCTGCCTTCTCTTGTG; reverse, GATGGTGATGGGTTTCCCGT |
172 bp |
| ALP | XM_006538500.1 | Forward, TCCCCGCAACAGATCTCCTA; reverse, AGGTGGAGTAGAGCCCTGAG |
157 bp |
| RUNX2 | NM_001145920.2 | Forward, AATTAACGCCAGTCGGAGCA; reverse, CACTTCTCGGTCTGACGACG |
70 bp |
| OCN | NM_001037939.2 | Forward, TCTATGACCTGCAGAGGGCT; reverse, ATAGCTCGTCACAAGCAGGG; |
223 bp |
| Col1α1 | NM_000088.3 | Forward, AGTGGTTTGGATGGTGCCAA; reverse, GCACCATCATTTCCACGAGC |
170 bp |
Abbreviations: ALP, alkaline phosphatase; Col1α1, collagen Iα1; GADPH, glyceraldehyde-3-phosphate dehydrogenase; OCN, osteocalcin; PCR, polymerase chain reaction; RT-PCR, real-time polymerase chain reaction; RUNX2, runt-related transcription factor 2.