Fig. 2.

Hepatic iron accumulation, plasma iron levels, and transferrin saturation are not affected by liver-specific inactivation of Dmt1 in Hfe knockout (Hfe^−/−) mice. (A) Hepatic non-heme iron concentrations were determined colorimetrically after acid digestion of tissues. (B) Plasma iron and (C) transferrin saturation were determined by using standard methods. Values represent mean ± SE, n=6. Means without a common superscript differ significantly (P < 0.05). (D) Histological examination of iron loading in the liver by using Perls’ Prussian blue to stain for iron. Branches of the portal vein (P) are indicated. All analyses were performed on samples from 16-week-old mice.