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. 2014 Dec 12;22(7):1158–1169. doi: 10.1038/cdd.2014.205

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MiR-294/302 suppresses and let-7 promotes apoptosis in ESCs. (a) Apoptosis analysis by Annexin V and propidium iodide staining in Dgcr8−/− ESCs mock transfected, transfected with let-7c, or co-transfected with let-7c and miR-294. Shown are mean±S.D., n=3. (b) Western blotting analysis of activated CASP3. Data were normalized to mock-transfected Dgcr8−/− ESCs. Shown are mean±range. n=2. (c) qRT-PCR analysis of regulators of apoptosis. β-Actin gene was used as a control. Data were normalized to the mRNA level of mock-transfected Dgcr8−/− ESCs. Shown are mean±S.D., n=3