Figure 2.

Potency test by mixed lymphocyte reaction (MLR). Dendritic cell functionality was evaluated via one-way MLR. ³H-thymidine incorporation (A) or colorimetric variation of MTS reagent (B) was used to detect cell proliferation. All mature dendritic cells (mDCs) analyzed were potent stimulatory cells by both ³H-thymidine and MTS analysis. The comparison between D1 and D2 mDCs showed that there are no differences in their ability to induce allogeneic MLR. A total of 54 preparations were tested with ³H-thymidine incorporation, and 22 of these also were tested with MTS incorporation. Abbreviations: D1, DENDR1; D2, DENDR2; SI, stimulation index.