Table 1.
Expression of IL-6, TNF-α and IL-17a in lung tissues of mice treated with vehicle control, NNK, LPS, NNK + LPS or NNK + LPS and I3C and/or Sila
| Vehicle control (n = 3) | NNK (n = 3) | LPS (n = 3) | NNK/LPS (n = 3) | NNK/LPS/Sil (n = 3) | NNK/LPS/I3C (n = 3) | NNK/LPS/Sil/ I3C (n = 3) | |
|---|---|---|---|---|---|---|---|
| IL-6 | 1.00±0.09 | 0.51±0.14 | 4.17±1.48b | 3.61±1.20b | 7.03±2.63 | 3.33±2.15 | 0.45±0.22c |
| TNF-α | 1.00±0.18 | 0.84±0.33 | 14.77±3.36b | 7.90±3.48b | 14.83±4.39 | 12.70±8.29 | 1.79±0.53c |
| IL-17a | 1.00±0.32 | 1.07±0.24 | 71.70±40.92b | 100.19±61.13b | 74.98±40.23 | 42.81±27.94 | 11.71±8.98 |
aFemale A/J mice were treated with NNK and/or LPS as described in the materials and methods section and maintained on control or I3C and/or Sil-supplemented diet. At week 25, mice were euthanized with an overdose of carbon dioxide, lung tissue harvested, and normal lung tissue or dissected tumors were kept in RNAlater and used for QRT-PCR-based analysis of IL-6, TNFα, and IL17a expression. Levels of the cytokines in the NNK, LPS and NNK + LPS groups were compared with that of the control group using Kruskal-Wallis test. Likewise, levels of the cytokines in the NNK + LPS + I3C and/or Sil groups were compared with that of the NNK + LPS group.
b P < 0.05, compared to vehicle control.
c P < 0.05, compared to the NNK + LPS group.