Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 15;5:14086. doi: 10.1038/srep14086

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Endogenous FXR target gene expression in HepG2 cells stably overexpressing GFP (HepG2-GFP; white bars) or GFP-FXR (HepG2-GFP-FXR; black bars). (A) Cells were treated in triplicate with DMSO, 1 μM GW4064, 10 μM MF, 5 ng/ml TNFα, GW4064 plus TNFα, or TNFα plus MF for 24 hours. IL8, MCP-1, and CXCL2 mRNA expression was analyzed by qRT-PCR in duplicate. (B) HepG2-GFP and HepG2-GFP-FXR cells were treated with DMSO, 1 μM GW4064 or 10 μM MF in triplicate for 24 hours. SHP, FGF19, KNG1, SDC1 and ICAM1 mRNA expression was analyzed by qRT-PCR in duplicate. Each bar represents mean ± SD. (C,D) Small intestine derived organoids from 3WT and FXR−/− mice were treated with DMSO, 1 μM GW4064, 10 μM MF, 5 ng/ml TNFα, GW4064 plus TNFα, or TNFα plus MF for 24 hours. mRNA expression of each organoid line was analyzed by qRT-PCR in duplicate. Each bar represents mean ± SEM.