Table 2. Primers used for qRT-PCR analysis and dsRNA synthesis.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| PxRyR | CGCCAACAAGATGAGTGAGA | CCCGGTGTCGATGTAGTCTT |
| L32 | ATCCGCCATCAGTCCGACCG | GGCTGAACCGTAACCAATGTTG |
| miR-7a | TGCCGACTAGTGATTTTGTTGTT | GAATCGAGCACCAGTTACGC |
| miR-8519 | CGGCGAAAATGTAATGATTTC | GAATCGAGCACCAGTTACGC |
| U6 | CGCAAGGATGACACGCAA | GAATCGAGCACCAGTTACGC |
| dsRyR | taatacgactcactataggg-TGTGAATTTCTGCGAAGACG | taatacgactcactataggg-TCATCCCCACTATGCTCTCC |
| dsDicer-1 | taatacgactcactataggg-AGATGGAACCTTTGTGGCAG | taatacgactcactataggg-CGGTTATGATCCATTTTGGG |
| dsEGFP | taatacgactcactataggg-CAGTGCTTCAGCCGCTAC | taatacgactcactataggg-GTTCACCTTGATGCCGTTC |
Note: Small letters are the sequence of T7 promotor.