SDS-PAGE of purified β -galactosidase from Lactobacillus plantarum. The enzyme was electrophoresed through 12 % acrylamide gel and stained with Commassie Brilliant Blue G-250. Lane 1, crude extract; Lane 2, ultra filtration; Lane 3, gel permeation fraction 4; Lane 4, gel permeation fraction 5; Lane 5, protein molecular mass marker (20 kDa to 205 kDa)