Table 2.
Purification steps | Volume (ml) | Total enzyme activity(IU) | Total protein (mg) | Specific activity(IUmg−1) | Purification factor | Yield |
---|---|---|---|---|---|---|
Crude extract (after sonication) | 30 | 3360 | 63 | 53.33 | 1 | 100 |
Ultrafiltration | 10 | 930.5 | 16.2 | 57.438 | 2.695 | 83.08 |
Sephadex G100 | 1 | 34.07 | 0.237 | 143.755 | 7.234 | 30.41 |