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. 2015 Jan 20;4(4):248–255. doi: 10.1080/21623945.2015.1007823

Figure 1.

Figure 1.

Gene expression during adipose differentiation of 3T3-F442A cells. (A) Adipose conversion shown by lipid staining with Oil Red O. Staurosporine (St) at low concentrations [10–12 nM] induced adipose differentiation in 3T3-F442A cells and this effect was augmented by Dexamethasone (Dex) at [250 nM]. Dex alone did not induce adipose differentiation; (N-Ad, non-adipogenic medium). (B) mRNA expression of adipogenic genes Cebpb, Pparg2, Cepba, Klf4 and Klf5 during adipose differentiation of 3T3-F442A cells treated with St-Dex. (C) Expression of Klf4 at 2h, Cebpb at 4h,and Klf5 at 3h, which are the times of their highest expression.