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Forced expression of Klf4 does not increase Cebpb and Klf5 expression. (A) Adipose conversion shown by lipid staining with Oil Red O. (B) Expression of the adipogenic genes Klf4, Cebpb, Klf5 and Pparg2 in cultures transfected with pCMVSport6 empty vector (pS-empty) and pCMVSport6Klf4 (pS-Klf4) in cells cultured in non-adipogenic conditions. (C) Representative western blot showing the amounts of KLF4 and using GDI protein as loading control.
