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. 2015 Jan 20;4(4):248–255. doi: 10.1080/21623945.2015.1007823

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Figure 3. — GSK3β activity and retinoic acid affect gene expression during adipose differentiation. (A) Adipose conversion shown by lipid staining with Oil Red O. GSK3β inhibitor (SB415286) at [100μM] or retinoic acid at [10μM]. (B and C) Expression of Pparg2, Cebpa, Klf4, Cebpb and Klf5 in 3T3F442A St-Dex cultures treated with SB415286 (black) or retinoic acid (blue). (D) Adipose conversion shown by lipid staining with Oil Red O in cultures treated with Cycloheximide (CHX) at [15μM]. (E) Expression of Klf4, Cebpb, and Klf5 in cultures treated with or without CHX for 8 h in St-Dex induced cultures.