Isolation and characterization of human adipose tissue from various depots. (A) Schematic of procedure for isolation of adipose tissue and preadipocytes from adult human subcutaneous and omental as well as fetal interscapular sites. (B) RT-PCR of RNA isolated from adult human subcutaneous (sub), fetal interscapular (fiBAT) and adult human omental (ome) adipose tissue. UCP-1, uncoupling protein-1; ZIC1, zinc finger of the cerebellum1; PGC1-α, peroxisome proliferator-activated receptor gamma co-activator 1-α; PPAR-α, peroxisome proliferator-activated receptor α; LHX, LIM homeobox; PPAR-γ, peroxisome proliferator-activated receptor gamma.