Table 1.
Microarray based evaluation and selection of putative reference genes
| Gene Title | Gene Symbol* | PR-BAT CV | PT-BAT CV | CV score** |
|---|---|---|---|---|
| Commonly used reference genes | ||||
| Beta-2-microglobulin | B2M | 0.075 | 0.111 | 0.0084 |
| Ubiquitin C | UBC | 0.065 | 0.140 | 0.0090 |
| Ribosomal protein, large, P0 | RPLP0 | 0.101 | 0.117 | 0.0118 |
| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | YWHAZ | 0.095 | 0.132 | 0.0125 |
| Peptidylprolyl isomerase A (cyclophilin A) | PPIA | 0.127 | 0.110 | 0.0140 |
| Low density lipoprotein receptor-related protein 10 | LRP10 | 0.100 | 0.176 | 0.0176 |
| Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa | POLR2A | 0.083 | 0.215 | 0.0179 |
| Phosphoglycerate kinase 1 | PGK1 | 0.163 | 0.136 | 0.0222 |
| Actin, β | ACTB | 0.101 | 0.239 | 0.0242 |
| TATA box binding protein | TBP | 0.145 | 0.179 | 0.0259 |
| Importin 8 | IPO8 | 0.168 | 0.171 | 0.0287 |
| Glucuronidase, β | GUSB | 0.138 | 0.249 | 0.0344 |
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 0.152 | 0.283 | 0.0429 |
| Hydroxymethylbilane synthase | HMBS | 0.267 | 0.240 | 0.0642 |
| Hypoxanthine phosphoribosyltransferase 1 | HPRT1 | 0.274 | 0.417 | 0.1144 |
| Transferrin receptor (p90, CD71) | TFRC | 0.382 | 0.530 | 0.2022 |
| 18S rRNA | 18S | n.a. | 0.245 | n.a. |
| PR-BAT dataset selected putative reference genes | ||||
| HECT, UBA and WWE domain containing 1 | HUWE1 | 0.044 | 0.082 | 0.0036 |
| Guanine nucleotide binding protein (G protein), β polypeptide 2 | GNB2 | 0.046 | 0.105 | 0.0048 |
| Family with sequence similarity 96, member B | FAM96B | 0.063 | 0.090 | 0.0057 |
| Proteasome (prosome, macropain) subunit, β type, 2 | PSMB2 | 0.061 | 0.097 | 0.0059 |
| Tumor protein, translationally-controlled 1 | TPT1 | 0.064 | 0.098 | 0.0063 |
| Guanine nucleotide binding protein (G protein), β polypeptide 1 | GNB1 | 0.060 | 0.105 | 0.0063 |
| Transmembrane protein 248 | TMEM248 | 0.046 | 0.151 | 0.0069 |
| Nuclear receptor binding protein 1 | NRBP1 | 0.053 | 0.136 | 0.0072 |
| SCY1-like 1 (S. cerevisiae) | SCYL1 | 0.056 | 0.131 | 0.0074 |
| ADP-ribosylation factor 1 | ARF1 | 0.062 | 0.123 | 0.0076 |
| PT-BAT data set selected putative reference genes | ||||
| Ring finger protein 1 | RING1 | 0.065 | 0.069 | 0.0045 |
| Family with sequence similarity 96, member B | FAM96B | 0.063 | 0.090 | 0.0057 |
| Proteasome (prosome, macropain) subunit, β type, 2 | PSMB2 | 0.061 | 0.097 | 0.0059 |
| Guanine nucleotide binding protein (G protein), β polypeptide 1 | GNB1 | 0.060 | 0.105 | 0.0063 |
| GNAS complex locus | GNAS | 0.081 | 0.091 | 0.0074 |
| ADP-ribosylation factor 1 | ARF1 | 0.062 | 0.123 | 0.0076 |
| Ring finger protein 1 | RING1 | 0.065 | 0.127 | 0.0082 |
| RNA binding protein S1, serine-rich domain | RNPS1 | 0.079 | 0.110 | 0.0087 |
| Transcription factor 25 (basic helix-loop-helix) | TCF25 | 0.077 | 0.115 | 0.0089 |
| ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) | ELAVL1 | 0.073 | 0.122 | 0.0089 |
*Gene symbols marked in bold text were selected for qPCR analysis.
**CV-score is the product of the CV values from the PT-BAT and PR-BAT datasets.
n.a., not available.