Fig. 3.
Cross-reactivity of VHH clones with human mEH, rat sEH, mouse sEH, and denatured human sEH. Rabbit polyclonal anti-human sEH antibody (4.5 μg/mL) as capture antibody, VHH A1, A6, A9, B1, B3, B4, B6, B7, B13 and B15 (2 μg/mL) as detector antibody and anti-HA tag-HRP (1:5000) as secondary antibody. Error bars are standard deviations of the mean of three well replicates.