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Estimated concentration of human sEH in spiked samples. The native and denatured human sEH (100, 200, 300 and 500 ng/mL) were spiked into sEH null mice liver cytosol. The native human sEH (100, 200, 300 and 500 ng/mL) with 1 μM 1728 inhibitor was spiked into PBS. Each sample was detected three times.
