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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2015 Jul 31;407(24):7275–7283. doi: 10.1007/s00216-015-8889-6

Table 2. Performance of 10 VHHs in the sandwich ELISA assay.

VHH clones SC50*
(ng/mL)		 Linear
range
(ng/mL)		 VHH clones SC50*
(ng/mL)		 Linear
range
(ng/mL)
A1 246 86-700 B4 373 123-1121
A6 515 151-1750 B6 1481 292-7514
A9 268 104-688 B7 356 113-1127
B1 341 108-1084 B13 463 141-1515
B3 225 74-684 B15 179 100-321

Sandwich ELISA conditions: Rabbit polyclonal anti-human sEH antibody (4.5 μg/mL) as capture antibody, VHHs (2 μg/mL) as detector antibody and anti-HA tag-HRP (1:5000) as secondary antibody. Error bars are standard deviations of the mean of three well replicates.

*

The 50% saturation of the signal (SC50).