Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2015 Jul 31;407(24):7275–7283. doi: 10.1007/s00216-015-8889-6

Table 3. The concentration of sEH in the S9 fraction of pooled (4–50 persons) human tissues.

Human
tissues		 sEH (nM)
estimated from
activity#
Mean ± SD		 estimated from
Western-blot#
Mean ± SD		 estimated from
ELISA
Mean ± SD
Liver 420 ± 89 500± 150 386 ± 48
Kidney 44 ± 3 80 ± 15 44.4 ± 1.6
Lung (N.S.*) 3.0 ± 0.3 22 ± 6 6.1 ± 0.3
Lung (S.*) 2.8 ± 0.3 21 ± 4 5.9 ± 0.1

Concentrations estimated from the specific activity using [3H] t -DPPO as substrate ([S]final = 50 μM), by Western blot and sandwich ELISA using recombinant purified human sEH as a standard. Results are average ± SD (n = 3).

*

N.S. nonsmoker; S. smoker.

#

Data from reference [39].