Table 4. Effect VHH on human sEH enzymatic activities.
| VHH clones | Concentration of VHH (μM) |
Remaining activity |
|
|---|---|---|---|
| EH activity (%) |
Phosphatase activity (%) |
||
| A1 | 0.71 | 66.4 ± 1.1 | 103 ± 4 |
| A6 | 0.44 | 87.6 ± 2.7 | 66.9 ± 3.0 |
| A9 | 0.75 | 94.7 ± 1.6 | 94.4 ± 7.3 |
| B1 | 1.02 | 93.6 ± 2.8 | 107 ± 1 |
| B3 | 1.11 | 124 ± 6 | 108 ± 3 |
| B4 | 0.49 | 95.4 ± 2.2 | 103 ± 5 |
| B6 | 0.51 | 96.6 ± 2.8 | 96.0 ± 5.1 |
| B7 | 2.35 | 83.2 ± 2.3 | 111 ± 8 |
| B13 | 1.76 | 85.4 ± 3.9 | 146 ±7 |
| B15 | 1.89 | 81.5 ± 3.6 | 133 ± 10 |
EH activity: Human sEH= 1nM, CMNPC as substrate=5 μM
Phosphatase activity: Human sEH= 2 nM, AttoPhos as substrate [S]=25 μM
Results are the average and standard deviation of triplicate determinations.