Figure 1. Expression of FLT1P1 in human CRC cell lines.
A In the human genome, FLT1/VEGFR1 is located at chromosome 13q12, whereas FLT1P1 resides at chromosome 3p21.3. FLT1P1 is an intron-free processed pseudogene. It is homologous to the VEGFR1 cDNA from exon 20 to 30.
B Phylogenetic analysis of FLT1P1 showing that human FLT1P1 is closely related to chimpanzee orthologs.
C Three CRC cell lines (HCP1, SW480, and DLD1) expressed FLT1P1 ncRNA at variable basal levels according to a custom TaqMan qPCR assay.
D HCP1, SW480, and DLD1 cells had medial to high levels of VEGFR1 mRNA expression according to a TaqMan qPCR assay.
