Table 1.
Real-time PCR primer sequences
| Gene | Sequence (5′–3′) | Annealing temp (°C) | ID | |
|---|---|---|---|---|
| FASN | Sense | GGAATGGGAAGACACCTATGGA | 62 | GenBank NM_004104.4 |
| Antisense | AGAGAGAGCTCAGATACGTTGAC | |||
| PPARG | Sense | TCTCTCCGTAATGGAAGACC | 62 | GenBank L40904.2 |
| Antisense | GCATTATGAGACATCCCCAC | |||
| ACTB | Sense | CCATCATGAAGTGTGACGTTG | 62 | GenBank NM_001101.3 |
| Antisense | ACAGAGTACTTGCGCTCAGGA | |||
| PPARG Hydrolysis Probe | Sense | GATGTCTCATAATGCCATCAGGTT | 60 | RTPrimerDB ID: 5471 |
| Antisense | GGATTCAGCTGGTCGATATCACT | |||
| Probe | CCAACAGCTTCTCCTTCTCGGCCTG | |||
| ACTB Hydrolysis Probe | Sense | TCCTTCCTGGGCATGGAG | 60 | RTPrimerDB ID: 2418 |
| Antisense | AGGAGGAGCAATGATCTTGATCTT | |||
| Probe | CCTGTGGCATCCACGAAACTACCTTC | |||