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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Exp Eye Res. 2015 Aug 4;139:97–107. doi: 10.1016/j.exer.2015.07.018

Figure 3. Measurement of HA concentration by ELISA.

Figure 3

TM cells were treated for 24 hours with TNFα, IL-1α or TGFβ2 or mechanical stretch and the HA levels in serum-free media and RIPA cell extracts was quantitated by a competitive ELISA assay. HA levels in RIPA lysates and media are presented as a percentage of the control. N=6; *, P<0.05 by ANOVA.