Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Exp Eye Res. 2015 Aug 4;139:97–107. doi: 10.1016/j.exer.2015.07.018

Figure 5. TSG-6 in TM cells.

Figure 5

TSG-6 (green) was colocalized with HAbp (red) in untreated PTM cells (A–C) and in cells treated for 3 days with TNFα (D–F). Merged images are shown (C, F). Confocal acquisition settings were maintained between untreated control and TNFα-treated TM cells. DAPI was used to stain the nuclei (blue). Scale bars = 20 µm. (G) Western immunoblots of TSG-6 in media in untreated and following treatment for 48 hours with TNFα, TGFβ2, IL-1α and mechanical stretch. The positions of TSG-6 complexed with HCs (~120 kDa) and a degradation product (80 kDa) are indicated. Equal amounts of protein were loaded into each lane for media. The cells on the left panel were cultured on plastic, whereas the cells in the right panel were cultured on collagen-coated silicone membrane. (I) Densitometry of TSG-6 levels in untreated and treated TM cells. N=3. * p < 0.05 by ANOVA.