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. Author manuscript; available in PMC: 2015 Sep 17.
Published in final edited form as: Biochemistry. 2015 Sep 2;54(36):5617–5631. doi: 10.1021/acs.biochem.5b00407

Figure 3.

Figure 3

Glycine supplementation ofthe culture medium significantly increases PPIX accumulation in HeLa cells expressing delAGTG. Plus (+) signs indicate samples in which the culture medium was supplemented with 100 glycine, and minus (−) signs indicate samples in which no glycine was added. The graph bars correspond to the results with wild-type hALAS2 (blue), delAT (green), delAGTG (pink), and Q548X (purple). Mean PPIX fluorescence values are representative of three separate experiments ± standard deviation (*p<0.05 and **p<0.01, Student’s t-test) [a.u., arbitrary units].