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Protein fluorescence changes associated with reaction of ALA with XLPP variants indicate an increase in the rate constant of product release (corresponding to the y-axis intercept as defined in Equation 3). Observed reaction rates for (A) wild-type hALAS2 (10 pM), (B) delAT (10 pM), and (C) delAGTG (10 pM) with ALA were determined by fitting the data to Equation 3.
