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Observed rates of quinonoid intermediate formation and decay under the single turnover reaction conditions described in Figure 3
| Parameter | Wild-type hALA2 (s−1) |
DelAT (s−1) |
DelAGTG (s−1) |
Q548X (s−1) |
|---|---|---|---|---|
| Q f | 6.6 ± 0.2 | 9.8 ± 0.4 | 11.1 ± 0.5 | 14.3 ± 0.6 |
| Q d | 0.70 ± 0.02 | 1.37 ± 0.04 | 1.97 ± 0.05 | 1.93 ± 0.07 |
