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Effects of isoflurane (ISO) and miR-21 knockout (KO) on NADH fluorescence in Langendorff-perfused hearts subjected to ischemia/reperfusion (I/R) injury. All hearts were stabilized for 30 min (baseline) and perfused with the buffer with or without ISO prior to 30 min of global ischemia followed by 2 h of reperfusion, as described in Figure 5. *P < 0.05 versus WT-I/R; #P < 0.05 versus WT-I/R+ISO (n = 7-9 hearts/group).
