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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Cancer Res. 2015 Jun 15;13(9):1306–1315. doi: 10.1158/1541-7786.MCR-15-0130

Fig. 6. Loss of IQGAP1 expression enhances TGF-β signaling.

Fig. 6

(A) The use of Ingenuity Pathway Analysis identified genes and pathways which had a biological connection to IQGAP1. The most dramatic association was found between IQGAP1 and TGFBR2, as well as its ligand TGF-β. (B) Loss of IQGAP1 from 253J cells leads to a statistically significant increase in TGFBR2 protein levels. The bar graph averages the results from 3 independent experiments. (C) The use of a TGF-β reporter assay revealed that loss of IQGAP1 from 253J cells leads to enhanced TGF-β signaling. Furthermore, this enhanced signaling was attenuated with the concurrent knockdown of TGFBR2. (D) Knockdown of TGFBR2 expression in 253J cells results in abrogation of the previously noted increase in soft agar colony formation that results from loss of IQGAP1. * p<0.05, ** p<0.01, *** p<0.001.