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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Mar 16;34(48):5912–5922. doi: 10.1038/onc.2015.41

Figure 4.

Figure 4

Ectopic expression of C/EBPβ suppresses LNCaP cell growth. (a) LNCaP PB-CEBPB and PB-TRE control cells were cultured for 5 days in doxycycline 0.5 μg/ml. Cell lysates were obtained and a representative Western blot analysis for C/EBPβ, p15INK4B and p16INK4A is shown. Numbers below the blots indicate the relative band density. (b) Phase-contrast images of LNCaP PB-TRE and PB-CEBPB cells after 5 days in culture with 0.5 μg/ml doxycycline. (c) 1.5E5 LNCaP PB-CEBPB or PB-TRE cells were seeded in media containing 0.5 μg/ml doxycycline. Cells were enumerated on day 3 and 5. (d) Cells were cultured under similar conditions and the Ki67 expression was analyzed after five days of doxycycline treatment. (e) The number of dead cells on day 5 was assessed by trypan blue exclusion. All graphs represent the average of three experiments, error bars: SEM; * p<0.01; ** p<0.02.