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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Gut. 2015 Mar 16;65(6):925–934. doi: 10.1136/gutjnl-2014-308416

Figure 2. DARPP-32 proteins up-regulate ANGPT2 expression and phosphorylate STAT3.

Figure 2

A–B) Western blot analysis of ANGTP2, phospho-AKT (S473) (P-AKT), phospho-P65 (S536), phospho-STAT3 (Y705) (P-STAT3) in AGS cells transiently overexpressing control, DARPP-32, or t-DARPP treated with or without AKT inhibitor MK2206 (5 μM, overnight). C) Immunofluorescence for DARPP-32 and phospho-STAT3 (Y705) in AGS cells transiently overexpressing DARPP-32 or control (200X). D) Quantitative data for phospho-STAT3 in panel C, *** P<0.001 (t test). E) Western blots confirming immunofluorescence data shown in panel C.