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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Gut. 2015 Mar 16;65(6):925–934. doi: 10.1136/gutjnl-2014-308416

Figure 3. DARPP-32 proteins-induced up-regulation of ANGPT2 expression is dependent on STAT3 pathway.

Figure 3

A) STAT3 reporter analysis in AGS cells stably expressing DARPP-32 (DP32) or control (pcDNA) with or without IL-6 treatment (200 ng/ml, 4 hours), * P<0.05, *** P<0.001 compared with AGS cells stably expressing pcDNA without IL-6 treatment (One way ANOVA). B) ANGPT2 mRNA level in AGS cells transiently overexpressing DARPP-32 proteins or control with or without STAT3 inhibitor AZD1480 (300 nmol/L, overnight), *** P<0.001 compared with AGS control cells without AZD1480 treatment (One way ANOVA). C) Western blot analysis of ANGPT2 in cell lysates and conditioned media of AGS cells transiently overexpressing DARPP-32 proteins or control, with control or STAT3 siRNA knockdown.