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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Gut. 2015 Mar 16;65(6):925–934. doi: 10.1136/gutjnl-2014-308416

Figure 4. DARPP-32 proteins enhance angiogenesis in gastric cancer in vitro.

Figure 4

A) Western blot data showing ANGPT2 protein levels in cell lysates or conditioned media from AGS cells transiently overexpressing DARPP-32 proteins or control. B) HUVEC tube formation analysis (left panel, 100X) and quantification data (right panel) using conditioned media from AGS cells transiently overexpressing DARPP-32 proteins or control, *** P<0.001 compared with AGS control cells (One way ANOVA). C) HUVEC tube formation analysis (left panel, 100X) and quantification data (right panel, One way ANOVA) using conditioned media from AGS cells transiently overexpressing DARPP-32 or control, with or without ANGPT2 binding antibody. D) Western blot analysis of ANGPT2 in conditioned media from the same cells as panel C.