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. Author manuscript; available in PMC: 2015 Sep 17.
Published in final edited form as: Cell. 2013 Dec 19;155(7):1464–1478. doi: 10.1016/j.cell.2013.11.034

Figure 6. Acute Administration of a High Fat Diet, and not the Presence of Obesity is Sufficient to Reorganize the Hepatic Circadian Clock.

Figure 6

(A) The number of hepatic metabolites affected by acute (three-day) high fat diet feeding. (B) Relative abundance of metabolites uracil, uridine, and NAD+ after acute HF diet. (0NC=ZT0, normal chow; 0HF=ZT0, high fat). (C) Bmal1 mRNA after three days on NC or HF diets (***-time, N=5). (D) Western blot of nuclear BMAL1 and p84 proteins. (E) BMAL1 targets (Nampt, Upp2 and Dbp), and PPARγ target (Cidec) after acute HF feeding. (Nampt, ***-time; *-diet; Upp2, ***-time; ***-diet, **-time/diet interaction; Dbp, ***-time; Cidec, *-time, N=5.) (F) CLOCK and BMAL1 occupancy at the Upp2 and Dbp promoter after 3-day feeding. (Clock occupancy, Upp2: **-time; **-diet *- interaction; CLOCK occupancy, Dbpi1: *-time; **- interaction; BMAL1 occupancy Dbpi1: *- interaction). (G) H3K4me3 marks at the Upp2 and Dbp promoters after 3-day feeding. (H3K4me3 at Dbpi1: *-time; **- interaction, N=4.) (H) Hepatic expression of Upp2, Dbp, and Pcx in animals removed from the HFD for two weeks (Upp2: ***-time, Dbp; ***-time, N=5). (I) BMAL1 occupancy at ZT12 at the promoters of Upp2 and Dbp in NC mice and mice withdrawn from the HF diet for two weeks. Error bars=SEM; ***-P<0.001, **-P<0.01, *-P<0.05.