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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Nat Methods. 2015 Apr 27;12(6):568–576. doi: 10.1038/nmeth.3365

Figure 4.

Figure 4

Utility of smFP_FLAG for array tomography. (a) Left: large-scale AT z-projections showing smFP_FLAG labeling of somatostatin-Cre interneurons with cell bodies in CA1 hippocampus stratum oriens (SO) and axonal projections in stratum lacunosum moleculare (SLM). Center: EGFP+ pyramidal cell signal from the same volume. Right: merged projection. (b) A series of 3 consecutive ultra-thin sections (100 nm) showing an smFP_FLAG-labeled varicosity (magenta) forming a putative synapsin (gray) immunopositive synapse onto a distal pyramidal cell dendrite (green) in SLM. Note the labeling consistency across serial sections for each channel. Bottom: zoomed images showing each channel from section two (white box), with arrows showing pixels overlaying between the synapsin and smFP_FLAG channels.