Fig. 4.

TP53INP1 was a functional target of miR-125b. a NSCLC cells from different patients (n = 3) were transfected with miR-125b inhibitors for 12 h and then assayed for their expression of the indicated genes using real time PCR. b NSCLC cells from one patient were transfected with miR-125b inhibitors for 48 h and then assayed for their expression of TP53INP1 using western blot. c NSCLC cells from different patients were transfected with TP53INP1 expression vector for 12 h and then assayed for their expression of TP53INP1 mRNA level. d, e NSCLC cells from different patients were co-transfected with miR-125 mimics and TP53INP1 expression vector, and then assayed for their adhesion activity (n = 4) and invasion (n = 5). f NSCLC cells from different patients were transfected with TP53INP1 siRNA or the control for 12 h and then assayed for their expression of TP53INP1. g, h NSCLC cells from different patients were co-transfected with miR-125 inhibitors and TP53INP1 siRNA, and then assayed for their adhesion activity (n = 4) and invasion (n = 5). Each dot represented the results from one patient