Fig 2. Src tyrosine kinase does not affect pHi recovery of hSMG acinar cells.
(A and B) The intracellular pH recovery patterns of hSMG acinar cells in the absence or presence of several concentrations of EIPA in HEPES-buffered solution (HBS) were measured and the pHi recovery rates were summarized. (C-F) The pHi recovery patterns of hSMG acinar cells following an NH4 +-pulse (blank bar) were recorded in a bicarbonate-buffered bath solution (BBS). The cells were pretreated for 20 min with 5 μM PP2, a Src tyrosine kinase inhibitor (grey trace) or incubated in normal BBS (black trace). The effects of treatment with EIPA and DIDS are shown using horizontal bars. (G) Summary of pHi recovery rates. The data are presented as the mean ± S.E.