Table 1.
Multiple reaction mode (MRM) transitions used for monitoring minor alkaloids and internal standards in tobacco samples.
Compound | Retention Time (min) | Quantitation MRM Transitions, m/z (Dwell (ms); Ionization energy (V)) | Confirmation MRM Transitions, m/z (Dwell (ms); Ionization energy (V)) |
---|---|---|---|
Nornicotine | 6.12 | 148.0 → 106.0 (90; 1) | 148.0 → 70.0 (90; 6) |
Myosmine | 6.20 | 146.1 → 118.1 (30; 22) | 146.1 → 91.0 (30; 35) |
Anabasine | 6.67 | 162.1 → 106.1 (20; 20) | 162.1 → 84.0 (20; 2) |
Anatabine | 6.98 | 160.0 → 145.1 (85; 6) | 160.0 → 82.0 (85; 7) |
Isonicoteine | 7.21 | 156.1 → 130.0 (85; 15) | 156.1 → 101.0 (85;37) |
D3-Nicotine (ISTD) | 5.04 | 165.1 → 136.1 (85; 25) | 165.1 → 87.1 (85; 8) |
D4-Nornicotine (ISTD) | 6.11 | 151.2 → 109.1 (30; 22) | 121.9 → 95.1 (30; 24) |