Table 3.

Method precision and accuracy for minor tobacco alkaloids spiked into blank matrix at three concentrations.

Analyte	Spike Level	Spike Concentration (μg/g)	Spike Accuracy (Recovery, %)	Spike Precision (CV, %)
Nornicotine	Low	468.4	102.4	0.9
	Medium	1124	112.4	3.1
	High	1991	101.6	3.3
Myosmine	Low	40.4	99.8	0.4
	Medium	96.9	104.7	1.1
	High	171.6	100.9	2.0
Anabasine	Low	332.5	106.2	1.1
	Medium	797.9	107.2	0.8
	High	1413	97.7	1.7
Anatabine	Low	406.3	106.0	1.1
	Medium	975.1	108.2	1.8
	High	1727	98.9	1.8
Isonicoteine	Low	60.6	108.7	1.2
	Medium	145.4	106.7	0.7
	High	257.4	96.8	1.6