Fig. 5.

Hsp70 release of MCF-7 and MDA-MB231 breast cancer cells after hypofractionated irradiation and/or hyperthermia. MCF-7 (a) or MDA-MB231 (b) breast cancer cells were treated with hypofractionated irradiation (4x4Gy or 6x3Gy) and/or hyperthermia (41,5 °C for 1 h). Ionizing irradiation was applied 4 h before or after hyperthermia on the first and last day of fractionation. 24 h after the last treatment, supernatants were analyzed for the amount of extracellular heat shock protein 70. Representative data of one out of four experiments, each performed at least in triplicates, are presented as mean ± SD. Gy: Gray; HT: hyperthermia; Hsp70: heat shock protein 70; w/o: mock treated control. *p <0,05, **p <0,01 compared to w/o; #p <0,05, ##p <0,01 compared to irradiated samples; +p <0,05, ++p <0,01 comparing 4x4Gy vs 6x3Gy treatments